What is the principle behind the Gram staining?

What is the principle behind the Gram staining?

The stain is retained as the cell walls of gram-positive bacteria are not as porous as those of gram-negative bacteria. If the bacteria is gram positive, the stain will remain in the smear. Otherwise, it will turn blue or purple.

How do you interpret gram stain results?

A Gram stain is purple. The stain reacts with bacteria to form a color. It can either turn purple, pink, or red depending on whether the bacteria is present in the sample. If the bacteria remains purple, they will be Gram-positive. Gram-negative bacteria are those that turn pink or red.

What does a Gram stain indicate?

A Gram Stain is a laboratory procedure that detects bacteria and sometimes fungi in samples taken from suspected infections.

What does it mean to have gram positive cocci in sputum?

Gram-positive diplococci (lancet or football-shaped) suggest Streptococcus pneumoniae. Clusters of Gram positive cocci suggest Staphylococcus aureus (B). The tiny Gram negative coccobacilli suggest Haemophilus influenzae.

What is meant by Gram-positive?

Gram-positive bacteria are bacteria with thick cell walls. These organisms are positive in a Gram stain test. This test uses a chemical dye to stain the cell walls of the bacterium. Gram-negative bacteria don’t have to hold the dye. Instead, they stain pink.

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What bacteria is Gram-positive cocci in pairs?

Streptococci are gram-positive cocci that grow in pairs or chains. Most pathogenic streptococci can be identified as facultative anaerobes.

Is pneumonia Gram-positive or negative?

Gram-positive pneumonia is a leading cause of morbidity and mortality throughout the world. The most common gram-positive pathogens that can cause pneumonia are Streptococcus pneumoniae, and Staphylococcus Aureus.

What antibiotics treat gram-negative pneumonia?

These antibiotics include cephalosporins (ceftriaxone-cefotaxime, ceftazidime, and others), fluoroquinolones (ciprofloxacin, levofloxacin), aminoglycosides (gentamicin, amikacin), imipenem, broad-spectrum penicillins with or without b-lactamase inhibitors (amoxicillin-clavulanic acid, piperacillin-tazobactam), and …

What is the Gram stain and shape of the cause of pneumonia?

Nocardia soteroides: The N asteroides bacillus is weakly gram positive and partially acid-fast. It forms thin, beaded filaments. It can cause lung abscesses or cavitations. It can also cause pleural erosion, which can lead to hematologic spreading of the organism.

What is the most common bacteria responsible for pneumonia?

Streptococcus pneumoniae, also known as pneumococcus, is a common cause of bacterial pneumococcus. But, it is not always possible for clinicians to determine which germ caused pneumonia. A person with community-acquired pneumonia is one who develops pneumonia in their own home and not in a hospital.

How does Streptococcus pneumoniae attack the body?

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Pneumococcal disease is caused by common bacteria (Streptococcus pneumoniae) that can attack different parts of the body. These bacteria can infect the lungs and cause pneumonia. They can also cause sepsis if they enter the bloodstream. Meningitis can be caused by them invading the brain covering.

Does Staphylococcus aureus cause pneumonia?

It’s the most common cause of skin and soft tissue infection, such as abscesses or boils, furuncles and cellulitis. Staph infections are usually not very serious. However, S. aureus may cause bloodstream infections, pneumonia, bone and joint infections, as well as bloodstream infections.

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