What is the difference between a leading and trailing strand in DNA replication?

What is the difference between a leading and trailing strand in DNA replication?

What is the difference between a leading and lagging DNA replication strand?

The double-stranded DNA is unzipped first by an enzyme called Helicase. Due to the divergent directions that the enzymes move on the lagging end of the DNA chain, it is only possible to synthesize small pieces. It is also known as the lagging string.

Why does each replication fork require both leading and lagging strand synthesis?

A. DNA synthesis is semi-conservative. Each template strand is synthesized using a fork-like structure. Each strand acts as a template. Because DNA polymerase synthesizes DNA in a 5-3′ direction, there is only one leading strand.

What is the difference between the lagging strand and the leading strand in a replication fork?

Both strands are synthesized at a replication fork in a 5′-3′ direction. The leading strand is synthesized continuously while the lagging one is synthesized in shorter pieces called Okazaki fragments.

Does the leading strand go towards the replication fork?

The leading strand is the first. This is the DNA parent strand that runs in the direction of the fork from 3′ to 5’. It can be reproduced continuously using DNA polymerase. The lagging strand is the other strand.

What is the function of 5 caps and 3 poly A tails?

The 5′ cap protects the nascent DNA from degrading and aids in ribosome binding during its translation. Once elongation has been completed, a poly (A) tail will be added to the pre-mRNA’s 3′ end.

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Are exons spliced?

The majority of splicing takes place between exons from a single transcript. However, trans-splicing can occur when exons from different pre-mRNAs get ligated together. The splicing process takes place in cellular machines called “spliceosomes”, in which the snRNPs and additional proteins are found.

How are splice sites recognized?

Components recognize sequences at intron ends known as splice site. The U1 small nuclear RNA (snRNP) initially binds the 5′ splice location (at the intron’s 5′ end), while the U2 auxiliary protein (U2AF3) binds the 3′ site (3, 4).

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